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Monoclonal antibody is made by immunizing animals using a synthetic peptide similar to residues bordering Ser296 of human Chk1 protein.
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Chk1 kinase acts downstream of ATM/ATR kinase and performs a vital purpose in DNA hurt checkpoint Handle, embryonic progress, and tumor suppression (1). Activation of Chk1 requires phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. Activation happens in response to blocked DNA replication and certain types of genotoxic anxiety (2). although phosphorylation at Ser345 serves to localize Chk1 to your nucleus next checkpoint activation (3), phosphorylation at Ser317 together with website-precise phosphorylation of PTEN allows for re-entry in to the cell cycle next stalled DNA replication (four). Chk1 exerts its checkpoint system to the cell cycle, partially, by regulating the cdc25 family of phosphatases.
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